rat small intestinal crypt epithelial cell line iec-6  (iCell Bioscience Inc)

Journal: Nutrients

Article Title: Vitamin D Receptor Protects against Radiation-Induced Intestinal Injury in Mice via Inhibition of Intestinal Crypt Stem/Progenitor Cell Apoptosis

doi: 10.3390/nu13092910

Figure Lengend Snippet: VDR deficiency suppresses intestinal epithelial regeneration and survival time of mice following IR. ( A ) Representative H&E images of intestine of WT and VDRKO mice at different times of 0, 5 h, 3 d (days), 4 d and 5 d after 12 Gy IR. Quantitative analysis of the number of regenerated crypts/1250 μm at different times after IR. n = 6 in each group. ( B ) Representative IHC staining of the expression of Ki67 of WT and VDRKO mice and quantification of Ki67 + cells/crypt at different times after IR. n = 6 in each group. Values are means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar, 200 μm. ( C ) Kaplan–Meier survival analysis of mice subjected to 12 Gy IR. The statistical analysis was performed by log-rank test. n ≥ 12 in each group.

Article Snippet: Rat small intestinal crypt epithelial cell line (IEC-6) was purchased from iCell Bioscience Inc. (Shanghai, China).

Techniques: Immunohistochemistry, Expressing